Fig 1: Knockdown of the clustered mitochondria homolog (CLUH) elicits perinuclear mitochondrial redistribution, elevated mitochondrial ROS generation, and enhances RSV virus production.(A–E) A549 cells were pretreated (48 hr) with siRNA (50 nM) for clustered mitochondria homolog (CLUH), or scrambled siRNA control (scr) followed by infection with RSV (MOI 1) for another 24 hr. (A) Immunoblot analysis for CLUH, or the control α-tubulin, as indicated (40 µg cell lysate protein/lane). (B and C) Immunostaining and R90% analysis were as per (Figure 1C and D). In all panels, scale bar = 10 µm. Results represent the mean ± SEM for n = 3 independent experiments, where each experiment analysed 25–30 cells per sample; ***p<0.001. (D) Cells were stained with Mitotracker Deep Red and the mitochondria-specific ROS probe FRR2 as per Figure 7A. The ratiometric output images of I(Ex514)/I(Ex488) were calculated as per Figure 7A. (E) FRR2 (I(Ex514)/I(Ex488)) was calculated for the mitochondrial regions defined by Mitotracker Deep Red staining in the I(Ex514)/I(Ex488) images such as those in D) using a custom CellProfiler pipeline as per Figure 7B. Results represent the mean ± SEM for n = 3 independent experiments, where each experiment analysed 25–30 cells per sample, ***p<0.001, **p<0.01.
Supplier Page from Thermo Fisher Scientific for CLUH Antibody